45350

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3231536..3232335 Replicon chromosome
Accession NZ_CP006636
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag PCN061_RS16280 Protein ID WP_000347273.1
Coordinates 3231871..3232335 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag PCN061_RS16275 Protein ID WP_001307405.1
Coordinates 3231536..3231871 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS16260 3227321..3228091 - 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -
PCN061_RS16265 3228107..3229441 - 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
PCN061_RS16270 3229816..3231387 + 1572 WP_001723936.1 galactarate dehydratase -
PCN061_RS16275 3231536..3231871 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
PCN061_RS16280 3231871..3232335 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
PCN061_RS16285 3232390..3233199 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
PCN061_RS16290 3233448..3234728 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
PCN061_RS16295 3234751..3235224 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
PCN061_RS16300 3235235..3236014 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
PCN061_RS16305 3236004..3236882 + 879 WP_001300474.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
PCN061_RS16310 3236900..3237334 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3222388..3232335 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T45350 WP_000347273.1 NZ_CP006636:3231871-3232335 [Escherichia coli PCN061]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T45350 NZ_CP006636:3231871-3232335 [Escherichia coli PCN061]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT45350 WP_001307405.1 NZ_CP006636:3231536-3231871 [Escherichia coli PCN061]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT45350 NZ_CP006636:3231536-3231871 [Escherichia coli PCN061]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References