103124

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4211376..4212175 Replicon chromosome
Accession NZ_CP028732
Organism Escherichia coli strain H3

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag AOY60_RS20460 Protein ID WP_000347273.1
Coordinates 4211711..4212175 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag AOY60_RS20455 Protein ID WP_001307405.1
Coordinates 4211376..4211711 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY60_RS20440 (4207161) 4207161..4207931 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
AOY60_RS20445 (4207947) 4207947..4209281 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
AOY60_RS20450 (4209656) 4209656..4211227 + 1572 WP_001273753.1 galactarate dehydratase -
AOY60_RS20455 (4211376) 4211376..4211711 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AOY60_RS20460 (4211711) 4211711..4212175 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AOY60_RS20465 (4212230) 4212230..4213039 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AOY60_RS20470 (4213288) 4213288..4214568 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
AOY60_RS20475 (4214591) 4214591..4215064 + 474 WP_001422480.1 PTS N-acetylgalactosamine transporter subunit IIB -
AOY60_RS20480 (4215075) 4215075..4215854 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
AOY60_RS20485 (4215844) 4215844..4216722 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
AOY60_RS20490 (4216740) 4216740..4217174 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4202228..4212175 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T103124 WP_000347273.1 NZ_CP028732:4211711-4212175 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T103124 NZ_CP028732:4211711-4212175 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT103124 WP_001307405.1 NZ_CP028732:4211376-4211711 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT103124 NZ_CP028732:4211376-4211711 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References