137625

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2589611..2590410 Replicon chromosome
Accession NZ_CP043197
Organism Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag FYA12_RS12630 Protein ID WP_000347273.1
Coordinates 2589611..2590075 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FYA12_RS12635 Protein ID WP_001307405.1
Coordinates 2590075..2590410 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA12_RS12605 2585787..2586344 - 558 Protein_2435 amidohydrolase family protein -
FYA12_RS12610 2586340..2586711 - 372 Protein_2436 PTS sugar transporter subunit IIC -
FYA12_RS12615 2586722..2587195 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
FYA12_RS12620 2587218..2588498 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
FYA12_RS12625 2588747..2589556 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FYA12_RS12630 2589611..2590075 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FYA12_RS12635 2590075..2590410 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FYA12_RS12640 2590559..2592130 - 1572 WP_001273753.1 galactarate dehydratase -
FYA12_RS12645 2592505..2593839 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
FYA12_RS12650 2593855..2594625 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2589611..2601285 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T137625 WP_000347273.1 NZ_CP043197:c2590075-2589611 [Escherichia coli O16:H48]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T137625 NZ_CP056852:c4677135-4676776 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCATGCCAGGAAAGATCTACAGGGAATGCCAGAACATGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGCGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACACATGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTGCCGGGAAGGCAACGCCCAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT137625 WP_001307405.1 NZ_CP043197:c2590410-2590075 [Escherichia coli O16:H48]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT137625 NZ_CP056852:c4676786-4676469 [Citrobacter freundii]
ATGTCACATGAAGTTGAAGCCAGTAGCGGAAATGTTTACGCCGATTTAGGCGTGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAACTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGTCCGCGCAGCTACTGGGAA
TGACGCAGCCTAAACTTTCCAACCTGCTACGTGGTCAGTTTCGTGGTATCAGTGAAGTAAAGATGCTCGAATGCCTGGTG
AGACTGGGTCGTGATGTAGAGATTGTTGTTGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References