107107

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 618390..619189 Replicon chromosome
Accession NZ_CP030111
Organism Escherichia coli strain MCJCHV-1

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag DPQ29_RS03165 Protein ID WP_000347275.1
Coordinates 618390..618854 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag DPQ29_RS03170 Protein ID WP_001307405.1
Coordinates 618854..619189 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPQ29_RS03130 613391..613825 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
DPQ29_RS03135 613843..614721 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
DPQ29_RS03140 614711..615490 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
DPQ29_RS03145 615501..615974 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
DPQ29_RS03150 615997..617277 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
DPQ29_RS03160 617526..618335 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
DPQ29_RS03165 618390..618854 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
DPQ29_RS03170 618854..619189 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
DPQ29_RS03175 619338..620909 - 1572 WP_001273752.1 galactarate dehydratase -
DPQ29_RS03180 621284..622618 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
DPQ29_RS03185 622634..623404 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 618390..630064 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T107107 WP_000347275.1 NZ_CP030111:c618854-618390 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T107107 NZ_CP030111:c618854-618390 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT107107 WP_001307405.1 NZ_CP030111:c619189-618854 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT107107 NZ_CP030111:c619189-618854 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References