219930

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3283078..3283877 Replicon chromosome
Accession NZ_CP084895
Organism Escherichia coli strain g74

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LHV63_RS16025 Protein ID WP_000347273.1
Coordinates 3283413..3283877 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LHV63_RS16020 Protein ID WP_001307405.1
Coordinates 3283078..3283413 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV63_RS16005 (3278863) 3278863..3279633 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
LHV63_RS16010 (3279649) 3279649..3280983 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LHV63_RS16015 (3281358) 3281358..3282929 + 1572 WP_001273753.1 galactarate dehydratase -
LHV63_RS16020 (3283078) 3283078..3283413 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LHV63_RS16025 (3283413) 3283413..3283877 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LHV63_RS16030 (3283932) 3283932..3284741 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LHV63_RS16035 (3284990) 3284990..3286270 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LHV63_RS16040 (3286293) 3286293..3286766 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LHV63_RS16045 (3286777) 3286777..3287148 + 372 Protein_3134 PTS sugar transporter subunit IIC -
LHV63_RS16050 (3287144) 3287144..3287701 + 558 Protein_3135 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3273930..3283877 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T219930 WP_000347273.1 NZ_CP084895:3283413-3283877 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T219930 NZ_CP116900:c1450726-1450349 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT219930 WP_001307405.1 NZ_CP084895:3283078-3283413 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT219930 NZ_CP116900:c1451150-1450773 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References