186022

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3791768..3792567 Replicon chromosome
Accession NZ_CP067299
Organism Escherichia coli strain ABW_A33

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag JJB24_RS18190 Protein ID WP_000347275.1
Coordinates 3792103..3792567 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJB24_RS18185 Protein ID WP_001307405.1
Coordinates 3791768..3792103 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB24_RS18170 (3787553) 3787553..3788323 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
JJB24_RS18175 (3788339) 3788339..3789673 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JJB24_RS18180 (3790048) 3790048..3791619 + 1572 WP_001273752.1 galactarate dehydratase -
JJB24_RS18185 (3791768) 3791768..3792103 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJB24_RS18190 (3792103) 3792103..3792567 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJB24_RS18195 (3792622) 3792622..3793431 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JJB24_RS18200 (3793680) 3793680..3794960 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJB24_RS18205 (3794983) 3794983..3795456 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJB24_RS18210 (3795467) 3795467..3796246 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
JJB24_RS18215 (3796236) 3796236..3797114 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JJB24_RS18220 (3797132) 3797132..3797566 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3782620..3792567 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T186022 WP_000347275.1 NZ_CP067299:3792103-3792567 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T186022 NZ_CP089154:1360072-1360260 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT186022 WP_001307405.1 NZ_CP067299:3791768-3792103 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT186022 NZ_CP089154:1359783-1360043 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References