181530

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3270359..3271158 Replicon chromosome
Accession NZ_CP064680
Organism Escherichia coli K-12 strain CETR1G40

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag IVL01_RS15865 Protein ID WP_000347273.1
Coordinates 3270694..3271158 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IVL01_RS15860 Protein ID WP_001307405.1
Coordinates 3270359..3270694 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IVL01_RS15845 3266144..3266914 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
IVL01_RS15850 3266930..3268264 - 1335 WP_195184510.1 MFS transporter -
IVL01_RS15855 3268639..3270210 + 1572 WP_001273753.1 galactarate dehydratase -
IVL01_RS15860 3270359..3270694 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IVL01_RS15865 3270694..3271158 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IVL01_RS15870 3271213..3272022 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IVL01_RS15875 3272271..3273551 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
IVL01_RS15880 3273574..3274047 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
IVL01_RS15885 3274058..3274429 + 372 Protein_3102 PTS sugar transporter subunit IIC -
IVL01_RS15890 3274425..3274982 + 558 Protein_3103 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3261211..3271158 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T181530 WP_000347273.1 NZ_CP064680:3270694-3271158 [Escherichia coli K-12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T181530 NZ_CP085947:c930004-929678 [Helicobacter pylori]
ATGCTGAAAATTAAGCACCATATCCTTTACAAAAAAGATTTTAAAAAGCTTGTTAAAAATGGGTTTGATGATAGCGTTTT
GAACAAAGTCATTCTAACCTTAAGAAAAAAAGAACCGCTAGATCCACAATTTCAAGATCATGCCCTAAAAGGCAAGTGGA
AACCTTTTAGGGAATGCCACATCAAGCCTGATATTTTGCTTGTGTATTTAGTGAAAGATGATGAGCTTATTTTGTTAAGG
TTAGGCAGTCATAGCGAGCTGTTTTATAAACCACCCATAACGCTTAAAAAGAACACAACAATCGCAGTTAACTCTAAACC
GCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT181530 WP_001307405.1 NZ_CP064680:3270359-3270694 [Escherichia coli K-12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT181530 NZ_CP085947:c930362-929991 [Helicobacter pylori]
ATGCCTAACACCACCGCCAAAAAAGACTACACAAAATACAGCGAAAAACAGCTTTTTAATTTAATCCATCAATTAGAGCG
AAAAATCAAAAAGATGCAAAATGATAGAGTTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGAACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGCTATTTG
GGGCACAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGTTAAATGGCGAAAACTTAGAAACTATTGAAGA
CTTTCACGCATGGGCAAATGAAGTGAAAAAGGAAGTGAATGCTGAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References