200936

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 598003..598802 Replicon chromosome
Accession NZ_CP073988
Organism Escherichia coli strain MB10

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KFU65_RS02920 Protein ID WP_000347273.1
Coordinates 598003..598467 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU65_RS02925 Protein ID WP_001307405.1
Coordinates 598467..598802 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU65_RS02890 (593004) 593004..593438 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU65_RS02895 (593456) 593456..594334 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU65_RS02900 (594324) 594324..595103 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU65_RS02905 (595114) 595114..595587 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU65_RS02910 (595610) 595610..596890 - 1281 WP_000681910.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU65_RS02915 (597139) 597139..597948 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU65_RS02920 (598003) 598003..598467 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU65_RS02925 (598467) 598467..598802 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU65_RS02930 (598951) 598951..600522 - 1572 WP_001273753.1 galactarate dehydratase -
KFU65_RS02935 (600897) 600897..602231 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU65_RS02940 (602247) 602247..603017 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 598003..609677 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T200936 WP_000347273.1 NZ_CP073988:c598467-598003 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T200936 NZ_CP098200:63569-63721 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGATTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200936 WP_001307405.1 NZ_CP073988:c598802-598467 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200936 NZ_CP098200:c63514-63457 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References