223495

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611447..612246 Replicon chromosome
Accession NZ_CP086480
Organism Escherichia coli strain Ec-050-T20-MAC

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag LNN46_RS02975 Protein ID WP_000347275.1
Coordinates 611447..611911 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LNN46_RS02980 Protein ID WP_001307405.1
Coordinates 611911..612246 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN46_RS02945 (606448) 606448..606882 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LNN46_RS02950 (606900) 606900..607778 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LNN46_RS02955 (607768) 607768..608547 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
LNN46_RS02960 (608558) 608558..609031 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LNN46_RS02965 (609054) 609054..610334 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LNN46_RS02970 (610583) 610583..611392 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LNN46_RS02975 (611447) 611447..611911 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LNN46_RS02980 (611911) 611911..612246 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LNN46_RS02985 (612395) 612395..613966 - 1572 WP_001273752.1 galactarate dehydratase -
LNN46_RS02990 (614341) 614341..615675 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LNN46_RS02995 (615691) 615691..616461 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 611447..623121 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T223495 WP_000347275.1 NZ_CP086480:c611911-611447 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T223495 NZ_CP118918:127349-127708 [Mycobacteroides chelonae]
ATGGAATCGACTAATCCCCGCCGGGGCGAACTGTGGCTGGTCGCCTTCGGCGCCGGCCGCCCCGGCGAACCCGCCAAACA
CCGGCCCGCCGTCATCGTGTCGGCTGACGAACTCCTCACCGGCGATCCAGATCCCACCGAACTCGTCGTGGTGATACCGG
TGTCGAGTTCGCGCACACCGACAAGCCTCCGGCCACCGATCACCCCCGATGAAGGCGTCGACACCAACAGCGTCGCAGTC
TGCCGGGCAGTCCGAGGCATCGCCCGAGGACGACTACTGCGCCACCTCGGAACGCTCAACAGACCCACGATGAGCCACAT
CGAGAAGTCCGTGGCCCTCATCCTCGGACTCGACCGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223495 WP_001307405.1 NZ_CP086480:c612246-611911 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223495 NZ_CP118918:127126-127362 [Mycobacteroides chelonae]
ATGTCTACACCTACAACAACGATCCGCGTACCGGTTACCACTCGTGATCGCCTCAATGCACAAGCCCGCGACCGCGGCAT
CTCCGTCGTCGCACTCCTCGACGAATGGTCCCACCGCGCCGAAAGGGACGCCGCCTTCGCCGAGGAACGCGCCGCCACCC
GCGCAGAAGCCGCCAACCCAGCCGTCATCGAGGAGGACCGCGACTGGGAACAGGCCGACGCCGATGGAATCGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References