194156

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4083775..4084574 Replicon chromosome
Accession NZ_CP070482
Organism Escherichia coli strain BTH101

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JWV29_RS19805 Protein ID WP_000347273.1
Coordinates 4083775..4084239 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JWV29_RS19810 Protein ID WP_001307405.1
Coordinates 4084239..4084574 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JWV29_RS19780 (4079951) 4079951..4080508 - 558 Protein_3867 amidohydrolase family protein -
JWV29_RS19785 (4080504) 4080504..4080875 - 372 Protein_3868 PTS sugar transporter subunit IIC -
JWV29_RS19790 (4080886) 4080886..4081359 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JWV29_RS19795 (4081382) 4081382..4082662 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JWV29_RS19800 (4082911) 4082911..4083720 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JWV29_RS19805 (4083775) 4083775..4084239 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JWV29_RS19810 (4084239) 4084239..4084574 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JWV29_RS19815 (4084723) 4084723..4086294 - 1572 WP_001273753.1 galactarate dehydratase -
JWV29_RS19820 (4086669) 4086669..4088003 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JWV29_RS19825 (4088019) 4088019..4088789 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4083775..4095449 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T194156 WP_000347273.1 NZ_CP070482:c4084239-4083775 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T194156 NZ_CP093373:443566-443850 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAATTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT194156 WP_001307405.1 NZ_CP070482:c4084574-4084239 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT194156 NZ_CP093373:443334-443576 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCCTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGAATTA
TGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References