135538

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3290228..3291027 Replicon chromosome
Accession NZ_CP042352
Organism Escherichia coli strain RM9513

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag FS836_RS16210 Protein ID WP_000347251.1
Coordinates 3290563..3291027 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag FS836_RS16205 Protein ID WP_001308975.1
Coordinates 3290228..3290563 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FS836_RS16190 (3286013) 3286013..3286783 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
FS836_RS16195 (3286799) 3286799..3288133 - 1335 WP_060615244.1 galactarate/glucarate/glycerate transporter GarP -
FS836_RS16200 (3288508) 3288508..3290079 + 1572 WP_064221457.1 galactarate dehydratase -
FS836_RS16205 (3290228) 3290228..3290563 + 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FS836_RS16210 (3290563) 3290563..3291027 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FS836_RS16215 (3291082) 3291082..3291891 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FS836_RS16220 (3292140) 3292140..3293420 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
FS836_RS16225 (3293443) 3293443..3293916 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
FS836_RS16230 (3293927) 3293927..3294706 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
FS836_RS16235 (3294696) 3294696..3295574 + 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
FS836_RS16240 (3295592) 3295592..3296026 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3279458..3291027 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T135538 WP_000347251.1 NZ_CP042352:3290563-3291027 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T135538 NZ_CP042352:3290563-3291027 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT135538 WP_001308975.1 NZ_CP042352:3290228-3290563 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT135538 NZ_CP042352:3290228-3290563 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References