171244

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4062351..4063150 Replicon chromosome
Accession NZ_CP060867
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag H9211_RS19640 Protein ID WP_000347275.1
Coordinates 4062686..4063150 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9211_RS19635 Protein ID WP_001307405.1
Coordinates 4062351..4062686 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS19620 (4058136) 4058136..4058906 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
H9211_RS19625 (4058922) 4058922..4060256 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9211_RS19630 (4060631) 4060631..4062202 + 1572 WP_001273752.1 galactarate dehydratase -
H9211_RS19635 (4062351) 4062351..4062686 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9211_RS19640 (4062686) 4062686..4063150 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9211_RS19645 (4063205) 4063205..4064014 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9211_RS19650 (4064263) 4064263..4065543 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9211_RS19655 (4065566) 4065566..4066039 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9211_RS19660 (4066050) 4066050..4066829 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9211_RS19665 (4066819) 4066819..4067697 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H9211_RS19670 (4067715) 4067715..4068149 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4053203..4063150 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T171244 WP_000347275.1 NZ_CP060867:4062686-4063150 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T171244 NZ_CP077867:2010962-2011228 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGTTATTATAGTAGACGTATCAATCACGAACATAGATTGGTCTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171244 WP_001307405.1 NZ_CP060867:4062351-4062686 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171244 NZ_CP077867:2010711-2010962 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACCTCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGAAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References