210480

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4237879..4238678 Replicon chromosome
Accession NZ_CP079991
Organism Escherichia coli strain KMS2005

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KYK86_RS20525 Protein ID WP_000347273.1
Coordinates 4238214..4238678 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KYK86_RS20520 Protein ID WP_001307405.1
Coordinates 4237879..4238214 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK86_RS20505 (4233664) 4233664..4234434 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
KYK86_RS20510 (4234450) 4234450..4235784 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KYK86_RS20515 (4236159) 4236159..4237730 + 1572 WP_001273753.1 galactarate dehydratase -
KYK86_RS20520 (4237879) 4237879..4238214 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KYK86_RS20525 (4238214) 4238214..4238678 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KYK86_RS20530 (4238733) 4238733..4239542 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KYK86_RS20535 (4239791) 4239791..4241071 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KYK86_RS20540 (4241094) 4241094..4241567 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KYK86_RS20545 (4241578) 4241578..4241949 + 372 Protein_4035 PTS sugar transporter subunit IIC -
KYK86_RS20550 (4241945) 4241945..4242502 + 558 Protein_4036 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4228731..4238678 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T210480 WP_000347273.1 NZ_CP079991:4238214-4238678 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T210480 NZ_CP079991:4238214-4238678 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT210480 WP_001307405.1 NZ_CP079991:4237879-4238214 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT210480 NZ_CP079991:4237879-4238214 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References