Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2627884..2628683 Replicon chromosome
Accession NZ_CP076318
Organism Escherichia coli strain Survcare045

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag KNN08_RS14255 Protein ID WP_000347275.1
Coordinates 2628219..2628683 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KNN08_RS14250 Protein ID WP_001307405.1
Coordinates 2627884..2628219 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN08_RS14235 (2623669) 2623669..2624439 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
KNN08_RS14240 (2624455) 2624455..2625789 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KNN08_RS14245 (2626164) 2626164..2627735 + 1572 WP_001273752.1 galactarate dehydratase -
KNN08_RS14250 (2627884) 2627884..2628219 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KNN08_RS14255 (2628219) 2628219..2628683 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KNN08_RS14260 (2628738) 2628738..2629547 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KNN08_RS14265 (2629796) 2629796..2631076 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KNN08_RS14270 (2631099) 2631099..2631572 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KNN08_RS14275 (2631583) 2631583..2632362 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KNN08_RS14280 (2632352) 2632352..2633230 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KNN08_RS14285 (2633248) 2633248..2633682 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2618736..2628683 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T205505 WP_000347275.1 NZ_CP076318:2628219-2628683 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T205505 NZ_CP101704:335215-335496 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT205505 WP_001307405.1 NZ_CP076318:2627884-2628219 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT205505 NZ_CP101704:335514-335804 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References