104960

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4221089..4221888 Replicon chromosome
Accession NZ_CP029115
Organism Escherichia coli strain AR435

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag CSC22_RS22460 Protein ID WP_000347275.1
Coordinates 4221424..4221888 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag CSC22_RS22455 Protein ID WP_001307405.1
Coordinates 4221089..4221424 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC22_RS22440 4216874..4217644 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
CSC22_RS22445 4217660..4218994 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
CSC22_RS22450 4219369..4220940 + 1572 WP_001273752.1 galactarate dehydratase -
CSC22_RS22455 4221089..4221424 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
CSC22_RS22460 4221424..4221888 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
CSC22_RS22465 4221943..4222752 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
CSC22_RS22475 4223001..4224281 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
CSC22_RS22480 4224304..4224777 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
CSC22_RS22485 4224788..4225567 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
CSC22_RS22490 4225557..4226435 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
CSC22_RS22495 4226453..4226887 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4211941..4221888 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T104960 WP_000347275.1 NZ_CP029115:4221424-4221888 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T104960 NZ_CP029115:4221424-4221888 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT104960 WP_001307405.1 NZ_CP029115:4221089-4221424 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT104960 NZ_CP029115:4221089-4221424 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References