255326

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 649956..650755 Replicon chromosome
Accession NZ_CP103529
Organism Escherichia coli strain 5270

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag M5T24_RS03165 Protein ID WP_000347275.1
Coordinates 649956..650420 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5T24_RS03170 Protein ID WP_001307405.1
Coordinates 650420..650755 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T24_RS03135 (644957) 644957..645391 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5T24_RS03140 (645409) 645409..646287 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5T24_RS03145 (646277) 646277..647056 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5T24_RS03150 (647067) 647067..647540 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5T24_RS03155 (647563) 647563..648843 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5T24_RS03160 (649092) 649092..649901 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5T24_RS03165 (649956) 649956..650420 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5T24_RS03170 (650420) 650420..650755 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5T24_RS03175 (650904) 650904..652475 - 1572 WP_001273752.1 galactarate dehydratase -
M5T24_RS03180 (652850) 652850..654184 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
M5T24_RS03185 (654200) 654200..654970 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 649956..661630 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T255326 WP_000347275.1 NZ_CP103529:c650420-649956 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT255326 WP_001307405.1 NZ_CP103529:c650755-650420 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References