258204

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4304444..4305243 Replicon chromosome
Accession NZ_CP104509
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag N5O74_RS21790 Protein ID WP_000347273.1
Coordinates 4304779..4305243 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N5O74_RS21785 Protein ID WP_001307405.1
Coordinates 4304444..4304779 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5O74_RS21770 (4300229) 4300229..4300999 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
N5O74_RS21775 (4301015) 4301015..4302349 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
N5O74_RS21780 (4302724) 4302724..4304295 + 1572 WP_001273753.1 galactarate dehydratase -
N5O74_RS21785 (4304444) 4304444..4304779 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N5O74_RS21790 (4304779) 4304779..4305243 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N5O74_RS21795 (4305298) 4305298..4306107 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N5O74_RS21800 (4306356) 4306356..4307636 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
N5O74_RS21805 (4307659) 4307659..4308132 + 474 WP_134235305.1 PTS N-acetylgalactosamine transporter subunit IIB -
N5O74_RS21810 (4308143) 4308143..4308922 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
N5O74_RS21815 (4308912) 4308912..4309790 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
N5O74_RS21820 (4309808) 4309808..4310242 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4295296..4305243 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T258204 WP_000347273.1 NZ_CP104509:4304779-4305243 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT258204 WP_001307405.1 NZ_CP104509:4304444-4304779 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References