43837

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3955905..3956704 Replicon chromosome
Accession NZ_AP027408
Organism Escherichia coli strain EC507 isolate EC507

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag QUE45_RS20700 Protein ID WP_000347251.1
Coordinates 3956240..3956704 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag QUE45_RS20695 Protein ID WP_001308975.1
Coordinates 3955905..3956240 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE45_RS20680 (3951690) 3951690..3952460 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
QUE45_RS20685 (3952476) 3952476..3953810 - 1335 WP_124037368.1 galactarate/glucarate/glycerate transporter GarP -
QUE45_RS20690 (3954185) 3954185..3955756 + 1572 WP_032181387.1 galactarate dehydratase -
QUE45_RS20695 (3955905) 3955905..3956240 + 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QUE45_RS20700 (3956240) 3956240..3956704 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QUE45_RS20705 (3956759) 3956759..3957568 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
QUE45_RS20710 (3957817) 3957817..3959097 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
QUE45_RS20715 (3959120) 3959120..3959593 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
QUE45_RS20720 (3959604) 3959604..3960383 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
QUE45_RS20725 (3960373) 3960373..3961251 + 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
QUE45_RS20730 (3961269) 3961269..3961703 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3945135..3956704 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T43837 WP_000347251.1 NZ_AP027408:3956240-3956704 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T43837 NZ_AP027408:3956240-3956704 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT43837 WP_001308975.1 NZ_AP027408:3955905-3956240 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT43837 NZ_AP027408:3955905-3956240 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References