276292

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 622577..623376 Replicon chromosome
Accession NZ_CP121356
Organism Escherichia coli strain rl0044

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag P9060_RS03050 Protein ID WP_000347273.1
Coordinates 622577..623041 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag P9060_RS03055 Protein ID WP_001307405.1
Coordinates 623041..623376 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P9060_RS03020 (617578) 617578..618012 - 435 WP_000948814.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
P9060_RS03025 (618030) 618030..618908 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
P9060_RS03030 (618898) 618898..619677 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
P9060_RS03035 (619688) 619688..620161 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
P9060_RS03040 (620184) 620184..621464 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
P9060_RS03045 (621713) 621713..622522 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
P9060_RS03050 (622577) 622577..623041 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
P9060_RS03055 (623041) 623041..623376 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
P9060_RS03060 (623525) 623525..625096 - 1572 WP_001273754.1 galactarate dehydratase -
P9060_RS03065 (625471) 625471..626805 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
P9060_RS03070 (626821) 626821..627591 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 622577..634139 11562


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T276292 WP_000347273.1 NZ_CP121356:c623041-622577 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT276292 WP_001307405.1 NZ_CP121356:c623376-623041 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References