157840

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4029351..4030150 Replicon chromosome
Accession NZ_CP053787
Organism Escherichia coli isolate J31

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HQN81_RS19475 Protein ID WP_000347273.1
Coordinates 4029686..4030150 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HQN81_RS19470 Protein ID WP_001307405.1
Coordinates 4029351..4029686 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQN81_RS19455 4025136..4025906 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
HQN81_RS19460 4025922..4027256 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HQN81_RS19465 4027631..4029202 + 1572 WP_001273753.1 galactarate dehydratase -
HQN81_RS19470 4029351..4029686 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HQN81_RS19475 4029686..4030150 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HQN81_RS19480 4030205..4031014 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HQN81_RS19485 4031263..4032543 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
HQN81_RS19490 4032566..4033039 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
HQN81_RS19495 4033050..4033829 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HQN81_RS19500 4033819..4034697 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HQN81_RS19505 4034715..4035149 + 435 WP_000948842.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4020203..4030150 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T157840 WP_000347273.1 NZ_CP053787:4029686-4030150 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T157840 NZ_CP069488:3831595-3831852 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT157840 WP_001307405.1 NZ_CP053787:4029351-4029686 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT157840 NZ_CP069488:3831864-3832415 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References