86156

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3333171..3333970 Replicon chromosome
Accession NZ_CP023834
Organism Escherichia coli strain 4/2-1

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag CRT37_RS15980 Protein ID WP_000347275.1
Coordinates 3333506..3333970 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag CRT37_RS15975 Protein ID WP_001307405.1
Coordinates 3333171..3333506 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT37_RS15960 3328956..3329726 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
CRT37_RS15965 3329742..3331076 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
CRT37_RS15970 3331451..3333022 + 1572 WP_001273752.1 galactarate dehydratase -
CRT37_RS15975 3333171..3333506 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
CRT37_RS15980 3333506..3333970 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
CRT37_RS15985 3334025..3334834 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
CRT37_RS15990 3335083..3336363 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
CRT37_RS15995 3336386..3336859 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
CRT37_RS16000 3336870..3337649 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
CRT37_RS16005 3337639..3338517 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
CRT37_RS16010 3338535..3338969 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3324023..3333970 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T86156 WP_000347275.1 NZ_CP023834:3333506-3333970 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T86156 NZ_CP023834:3333506-3333970 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT86156 WP_001307405.1 NZ_CP023834:3333171-3333506 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT86156 NZ_CP023834:3333171-3333506 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References