106602

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1592669..1593468 Replicon chromosome
Accession NZ_CP029741
Organism Escherichia coli strain AR_0085

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag AM464_RS08260 Protein ID WP_000347273.1
Coordinates 1593004..1593468 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag AM464_RS08255 Protein ID WP_001307405.1
Coordinates 1592669..1593004 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM464_RS08240 1588454..1589224 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
AM464_RS08245 1589240..1590574 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
AM464_RS08250 1590949..1592520 + 1572 WP_072778057.1 galactarate dehydratase -
AM464_RS08255 1592669..1593004 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AM464_RS08260 1593004..1593468 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AM464_RS08265 1593523..1594332 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AM464_RS08275 1594581..1595861 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
AM464_RS08280 1595884..1596357 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
AM464_RS08285 1596368..1597147 + 780 WP_000406208.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
AM464_RS08290 1597137..1598015 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
AM464_RS08295 1598033..1598467 + 435 WP_000948842.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1583634..1593468 9834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T106602 WP_000347273.1 NZ_CP029741:1593004-1593468 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T106602 NZ_CP029741:1593004-1593468 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT106602 WP_001307405.1 NZ_CP029741:1592669-1593004 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT106602 NZ_CP029741:1592669-1593004 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References