Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4293636..4294435 Replicon chromosome
Accession NZ_CP028741
Organism Escherichia coli strain A34

Toxin (Protein)


Gene name yhaV Uniprot ID -
Locus tag AOY87_RS21125 Protein ID WP_021568807.1
Coordinates 4293971..4294435 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag AOY87_RS21120 Protein ID WP_001307405.1
Coordinates 4293636..4293971 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY87_RS21105 (4289421) 4289421..4290191 - 771 WP_240765683.1 2-dehydro-3-deoxyglucarate aldolase -
AOY87_RS21110 (4290207) 4290207..4291541 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
AOY87_RS21115 (4291916) 4291916..4293487 + 1572 WP_001273741.1 galactarate dehydratase -
AOY87_RS21120 (4293636) 4293636..4293971 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AOY87_RS21125 (4293971) 4293971..4294435 + 465 WP_021568807.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AOY87_RS21130 (4294490) 4294490..4295299 - 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
AOY87_RS21135 (4295548) 4295548..4296828 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
AOY87_RS21140 (4296851) 4296851..4297324 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
AOY87_RS21145 (4297335) 4297335..4298114 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
AOY87_RS21150 (4298104) 4298104..4298982 + 879 WP_240765684.1 PTS N-acetylgalactosamine transporter subunit IID -
AOY87_RS21155 (4299000) 4299000..4299434 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4282761..4294435 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17808.19 Da        Isoelectric Point: 9.4947

>T103322 WP_021568807.1 NZ_CP028741:4293971-4294435 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTQETEETH

Download         Length: 465 bp

>T103322 NZ_CP028741:4293971-4294435 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCAAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT103322 WP_001307405.1 NZ_CP028741:4293636-4293971 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT103322 NZ_CP028741:4293636-4293971 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
ACCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References