Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3568427..3569226 Replicon chromosome
Accession NZ_CP067265
Organism Escherichia coli strain ABW_S38

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JJB13_RS17280 Protein ID WP_000347273.1
Coordinates 3568427..3568891 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJB13_RS17285 Protein ID WP_001307405.1
Coordinates 3568891..3569226 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB13_RS17250 (3563428) 3563428..3563862 - 435 WP_097480507.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JJB13_RS17255 (3563880) 3563880..3564758 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
JJB13_RS17260 (3564748) 3564748..3565527 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JJB13_RS17265 (3565538) 3565538..3566011 - 474 WP_001723937.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJB13_RS17270 (3566034) 3566034..3567314 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJB13_RS17275 (3567563) 3567563..3568372 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JJB13_RS17280 (3568427) 3568427..3568891 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJB13_RS17285 (3568891) 3568891..3569226 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJB13_RS17290 (3569375) 3569375..3570946 - 1572 WP_001723936.1 galactarate dehydratase -
JJB13_RS17295 (3571321) 3571321..3572655 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
JJB13_RS17300 (3572671) 3572671..3573441 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3568427..3580100 11673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T185992 WP_000347273.1 NZ_CP067265:c3568891-3568427 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T185992 NZ_CP089138:1050-1298 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT185992 WP_001307405.1 NZ_CP067265:c3569226-3568891 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT185992 NZ_CP089138:1295-1642 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References