118716

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2548711..2549510 Replicon chromosome
Accession NZ_CP035836
Organism Escherichia coli strain WS1933D

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag EXA03_RS12655 Protein ID WP_000347251.1
Coordinates 2549046..2549510 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EXA03_RS12650 Protein ID WP_001307405.1
Coordinates 2548711..2549046 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA03_RS12635 (2544496) 2544496..2545266 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
EXA03_RS12640 (2545282) 2545282..2546616 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
EXA03_RS12645 (2546991) 2546991..2548562 + 1572 WP_001273763.1 galactarate dehydratase -
EXA03_RS12650 (2548711) 2548711..2549046 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EXA03_RS12655 (2549046) 2549046..2549510 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EXA03_RS12660 (2549565) 2549565..2550374 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
EXA03_RS12665 (2550623) 2550623..2551903 + 1281 WP_000681906.1 tagatose-bisphosphate aldolase subunit KbaZ -
EXA03_RS12670 (2551926) 2551926..2552399 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EXA03_RS12675 (2552410) 2552410..2553189 + 780 WP_000406213.1 PTS N-acetylgalactosamine transporter subunit IIC -
EXA03_RS12680 (2553179) 2553179..2554057 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
EXA03_RS12685 (2554075) 2554075..2554509 + 435 WP_000948827.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2537941..2549510 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T118716 WP_000347251.1 NZ_CP035836:2549046-2549510 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T118716 NZ_CP035836:2549046-2549510 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT118716 WP_001307405.1 NZ_CP035836:2548711-2549046 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT118716 NZ_CP035836:2548711-2549046 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References