Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 612124..612923 Replicon chromosome
Accession NZ_CP103694
Organism Escherichia coli strain ST12468

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag M5T31_RS02975 Protein ID WP_000347273.1
Coordinates 612124..612588 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5T31_RS02980 Protein ID WP_001307405.1
Coordinates 612588..612923 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T31_RS02945 (607125) 607125..607559 - 435 WP_058905827.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5T31_RS02950 (607577) 607577..608455 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
M5T31_RS02955 (608445) 608445..609224 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5T31_RS02960 (609235) 609235..609708 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5T31_RS02965 (609731) 609731..611011 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5T31_RS02970 (611260) 611260..612069 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5T31_RS02975 (612124) 612124..612588 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5T31_RS02980 (612588) 612588..612923 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5T31_RS02985 (613072) 613072..614643 - 1572 WP_001723936.1 galactarate dehydratase -
M5T31_RS02990 (615018) 615018..616352 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
M5T31_RS02995 (616368) 616368..617138 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 612124..623682 11558


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T256195 WP_000347273.1 NZ_CP103694:c612588-612124 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT256195 WP_001307405.1 NZ_CP103694:c612923-612588 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References