Detailed information of TA system TA141371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 605445..606244
• Replicon: chromosome
• Accession: NZ_CP045190
• Organism: Escherichia coli strain NT1F31

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: GAG93_RS04050
• Protein ID: WP_000347273.1
• Coordinates: 605445..605909 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: GAG93_RS04055
• Protein ID: WP_001307405.1
• Coordinates: 605909..606244 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAG93_RS04020	600446..600880	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
GAG93_RS04025	600898..601776	-	879	WP_001300474.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
GAG93_RS04030	601766..602545	-	780	WP_156262346.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
GAG93_RS04035	602556..603029	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
GAG93_RS04040	603052..604332	-	1281	WP_096178318.1	tagatose-bisphosphate aldolase subunit KbaZ	-
GAG93_RS04045	604581..605390	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
GAG93_RS04050	605445..605909	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
GAG93_RS04055	605909..606244	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
GAG93_RS04060	606393..607964	-	1572	WP_001723936.1	galactarate dehydratase	-
GAG93_RS04065	608339..609673	+	1335	WP_001723935.1	galactarate/glucarate/glycerate transporter GarP	-
GAG93_RS04070	609689..610459	+	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020080 3504819..3516493 0.008 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021455 3265982..3277656 0.008 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068442 3158126..3169800 0.008 View NZ_CP020543 Escherichia coli C Genomic island TA075057 640607..652281 0.008 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285025 3504819..3516493 0.008	inside	Genomic island	-	-	605445..617119	11674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T141371 WP_000347273.1 NZ_CP045190:c605909-605445 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T141371 NZ_CP059955:2589820-2590395 [Pectobacterium brasiliense]
ATGAATCACTCCCCCTATCCTGTGGTACTTGATGCGTGTGTCATTTATCCATCTTTGCTACGGGATTTGCTCATGCATCT
TGGTTTAACAGGGCTATATCAGCCAAAGTGGACGGTACTAATTCAGGAAGAGTGGCAGCGAAATTTGTTGCTAAATAATG
CAGACGTAACGATGGATAAGCTCAGGAACATTGAGCATTTGATGAATAAGGCATTACCTGATGCCAATGTGACTGGGTTC
GAACAACTTATCCCTGCTCTGGTTTTACCTGATGCGGATGATCGACATGTATTGGCCGCTGCGATACGCGCTAATGCAGA
AGTTATCGTCACGGCAAATCTGAAAGATTTTCCAGCCACGTCACTTTCCGAATTTGGAGTGGAAGCCATGCACCCAGACG
AATTCATTGCCGATTTATTTGATCTCAATTCTGCACTGGTGCTTAAAGCCGTTCGAACTTCGCGAACAGCCTTCAGAAAG
CCTCCATTTGATGTGGACGAGTATCTGGATTGTCTCCTGCGTCAGGGGTTACCTATGACGGTAAAAGAACTGGATGCGTA
TCGAGCACTGATTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT141371 WP_001307405.1 NZ_CP045190:c606244-605909 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT141371 NZ_CP059955:2589359-2589820 [Pectobacterium brasiliense]
ATGAAAAATACTCCTCTAAATAGTTTGAGCCTTCCGGCTCCAAAAGAAATGGAGGCCGCTATCCGTGGACAAAGGCAGCT
TGCAACTTACCTGTCTACGAAGCTGGAAACACAGGAAATTTCGATTCAGGATGCGCACGACATTGCCCATCGGATCGAGT
TGCCTACCTCAGCACTCACGCTTTTGATGAGTATCCTTGGCGAACTGGCGGCGGGTAATGCTGTCCAGATTGTCCCTGTG
CAGGCTGAATTAACGACACAAGAAGCGGCAAATATTCTGAATGTTTCACGGCCACATATGGTGAAGCTTTTGGAAGAAGG
CCATCTTCCTTTCCATAAAACAGGTCGTCATCGCCGCGTGCTTTTTGCTGATTTGATGCAATACAAAGAACAGCGTGAAA
ATGAAAGTATGCAAGCCATGCAGGAGCTGACAGACCGAAGTCAGGAATTCGGGTTTTACTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7