162163

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 615158..615957 Replicon chromosome
Accession NZ_CP055259
Organism Escherichia coli strain AH62

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HU676_RS02950 Protein ID WP_000347273.1
Coordinates 615158..615622 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HU676_RS02955 Protein ID WP_001307405.1
Coordinates 615622..615957 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU676_RS02920 610159..610593 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HU676_RS02925 610611..611489 - 879 WP_001300474.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HU676_RS02930 611479..612258 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HU676_RS02935 612269..612742 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HU676_RS02940 612765..614045 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
HU676_RS02945 614294..615103 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HU676_RS02950 615158..615622 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HU676_RS02955 615622..615957 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HU676_RS02960 616106..617677 - 1572 WP_001723936.1 galactarate dehydratase -
HU676_RS02965 618052..619386 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
HU676_RS02970 619402..620172 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 615158..626830 11672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T162163 WP_000347273.1 NZ_CP055259:c615622-615158 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T162163 NZ_CP071626:c3711562-3711272 [Rhizobium leguminosarum]
ATGGCTGAACAGATTTTCGACTTTGATCCGGCTGAGATGCTTGATTCCGACGAGGCCATCGAAGCCTTTATCATCGAGGC
ATTGCAGACGGGAGACGCGAAATTCATCGCGTCCGCTCTTGGTGTCGTCGCGCGTGCCAAGGGCATGAGTAAGATCTCCA
GGGAAACAGGCCTTGCTCGCGAGCATCTTTATAAGGCACTGAGCGAAAACGGAAATCCCACCCTGGAGACCACGCTGGCG
GTCATGAAGGCAATCGGGCTTGAGCTGACGACCAAGCATCACGCGGTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT162163 WP_001307405.1 NZ_CP055259:c615957-615622 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT162163 NZ_CP071626:c3711860-3711555 [Rhizobium leguminosarum]
ATGTTCATCTTGAAGCAGACAGCTACTTTTCGAAAGTGGCACAAAAAATTGAAAGATGGAAAGGCCAAGGCGACGATTGC
CTTGCGTCTTCAGCGTCTCGCGACCGGGCATGCCGGCGACGCCTCACCGATCGGCGAGGGGCTGAGCGAGTTGCGCATTC
ACTATGGGCCTGGCTATCGGGTCTATTATTGTAAACGCGGAGAAACTATCATCGTGCTTCTCTGCGGCGGTGACAAAAGC
ACGCAGGAAACCGATATACGAACGGCAAAAAGAATCGCCTCGGAATGGAGCGACGATAATGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References