Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1845894..1846693 Replicon chromosome
Accession NZ_CP035884
Organism Escherichia coli strain LSN03-016011/A

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag EW995_RS08880 Protein ID WP_000347251.1
Coordinates 1846229..1846693 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EW995_RS08875 Protein ID WP_001307405.1
Coordinates 1845894..1846229 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW995_RS08860 (1841679) 1841679..1842449 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
EW995_RS08865 (1842465) 1842465..1843799 - 1335 WP_000599653.1 galactarate/glucarate/glycerate transporter GarP -
EW995_RS08870 (1844174) 1844174..1845745 + 1572 WP_001273761.1 galactarate dehydratase -
EW995_RS08875 (1845894) 1845894..1846229 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EW995_RS08880 (1846229) 1846229..1846693 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EW995_RS08885 (1846748) 1846748..1847557 - 810 WP_000072192.1 aga operon transcriptional regulator AgaR -
EW995_RS08890 (1847806) 1847806..1849086 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
EW995_RS08895 (1849109) 1849109..1849582 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EW995_RS08900 (1849593) 1849593..1850372 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
EW995_RS08905 (1850362) 1850362..1851240 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
EW995_RS08910 (1851258) 1851258..1851692 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1835124..1846693 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T119035 WP_000347251.1 NZ_CP035884:1846229-1846693 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T119035 NZ_CP035884:1846229-1846693 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT119035 WP_001307405.1 NZ_CP035884:1845894-1846229 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT119035 NZ_CP035884:1845894-1846229 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References