118653

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1433062..1433861 Replicon chromosome
Accession NZ_CP035826
Organism Escherichia coli strain 67-1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag EXA05_RS06895 Protein ID WP_000347251.1
Coordinates 1433397..1433861 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EXA05_RS06890 Protein ID WP_001307405.1
Coordinates 1433062..1433397 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA05_RS06875 (1428847) 1428847..1429617 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
EXA05_RS06880 (1429633) 1429633..1430967 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
EXA05_RS06885 (1431342) 1431342..1432913 + 1572 WP_001273763.1 galactarate dehydratase -
EXA05_RS06890 (1433062) 1433062..1433397 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EXA05_RS06895 (1433397) 1433397..1433861 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EXA05_RS06900 (1433916) 1433916..1434725 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
EXA05_RS06905 (1434974) 1434974..1436254 + 1281 WP_000681906.1 tagatose-bisphosphate aldolase subunit KbaZ -
EXA05_RS06910 (1436277) 1436277..1436750 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EXA05_RS06915 (1436761) 1436761..1437540 + 780 WP_000406213.1 PTS N-acetylgalactosamine transporter subunit IIC -
EXA05_RS06920 (1437530) 1437530..1438408 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
EXA05_RS06925 (1438426) 1438426..1438860 + 435 WP_000948827.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1422292..1433861 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T118653 WP_000347251.1 NZ_CP035826:1433397-1433861 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T118653 NZ_CP035826:1433397-1433861 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT118653 WP_001307405.1 NZ_CP035826:1433062-1433397 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT118653 NZ_CP035826:1433062-1433397 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References