Detailed information of TA system TA052524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4028811..4029610
• Replicon: chromosome
• Accession: NZ_CP010440
• Organism: Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: SH08_RS19925
• Protein ID: WP_000347273.1
• Coordinates: 4029146..4029610 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: SH08_RS19920
• Protein ID: WP_001307405.1
• Coordinates: 4028811..4029146 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SH08_RS19900	4024596..4025366	-	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-
SH08_RS19905	4025382..4026716	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
SH08_RS19915	4027091..4028662	+	1572	WP_001273753.1	galactarate dehydratase	-
SH08_RS19920	4028811..4029146	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
SH08_RS19925	4029146..4029610	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
SH08_RS19930	4029665..4030474	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
SH08_RS19935	4030723..4032003	+	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
SH08_RS19940	4032026..4032499	+	474	WP_001336162.1	PTS N-acetylgalactosamine transporter subunit IIB	-
SH08_RS19945	4032510..4032881	+	372	Protein_3776	PTS sugar transporter subunit IIC	-
SH08_RS19950	4032877..4033434	+	558	Protein_3777	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 Genomic island TA020042 3247092..3263092 0 View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020080 3504819..3516493 0.008 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021455 3265982..3277656 0.008 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068442 3158126..3169800 0.008 View NZ_CP020543 Escherichia coli C Genomic island TA075057 640607..652281 0.008 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285025 3504819..3516493 0.008	inside	Genomic island	-	-	4013610..4029610	16000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T52524 WP_000347273.1 NZ_CP010440:4029146-4029610 [Escherichia coli K-12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T52524 NZ_CP010440:4029146-4029610 [Escherichia coli K-12]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT52524 WP_001307405.1 NZ_CP010440:4028811-4029146 [Escherichia coli K-12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT52524 NZ_CP010440:4028811-4029146 [Escherichia coli K-12]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7