Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 692893..693692 Replicon chromosome
Accession NZ_CP061758
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag IEC98_RS03290 Protein ID WP_000347273.1
Coordinates 692893..693357 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IEC98_RS03295 Protein ID WP_001307405.1
Coordinates 693357..693692 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS03260 687893..688327 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IEC98_RS03265 688345..689224 - 880 Protein_646 PTS mannose/fructose/sorbose transporter family subunit IID -
IEC98_RS03270 689214..689993 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IEC98_RS03275 690004..690477 - 474 WP_001723937.1 PTS N-acetylgalactosamine transporter subunit IIB -
IEC98_RS03280 690500..691780 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
IEC98_RS03285 692029..692838 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IEC98_RS03290 692893..693357 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IEC98_RS03295 693357..693692 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IEC98_RS03300 693841..695412 - 1572 WP_001723936.1 galactarate dehydratase -
IEC98_RS03305 695787..697121 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
IEC98_RS03310 697137..697907 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 692893..705342 12449


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T172929 WP_000347273.1 NZ_CP061758:c693357-692893 [Escherichia coli O68:H12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T172929 NZ_CP080029:c4833249-4832815 [Streptomyces anulatus]
TTGATCATCGTCGCCGACACCTCGGCCCTCTACGCCGCTTTCGACGCCGCACAGACGGAGCACTCCGAGGCGGCCAGGAT
CCTGGAGAACGAGCGGCTGGCCATCTCCCCCCTGGTCGTCACCGAGCTGGACCACCTGGTCCATCGCGACCTGGGGTTCC
CGGCGGCCATGCAGGTGATGGAGGCATTGAATTCCCGTATGGGCGACGGGCAGTACCGCCTCGCCGAACTCAAGCTCGCG
GACCTGCGTACCGCCCAGGAAGTCCGGCAGAAGTACGAAGGTCTGCGGCTCGACCTGGCCGACGCGGTCGGAGTGGTCCT
CGCCGACCGCTACCGGACGGACCGCATCTTCACCTTGGACCAGCGCGACTTCCGGGCGATGTCACCACTGACCCCCGGAC
TGGACGCCTTCCGGATCCTGCCCGCCGACGGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT172929 WP_001307405.1 NZ_CP061758:c693692-693357 [Escherichia coli O68:H12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT172929 NZ_CP080029:c4833488-4833246 [Streptomyces anulatus]
ATGATAAAGACGACCGTGTACCTTCCGGAGGAACTGGAAGTGCGACTCGACGCCGAGTCCTCCGCGACCGGCGTCAGCAA
GGCCGAGCTCATCCGGCGGAGCATCGCCCTGCTGCTGGACAGCGCGGAGCGGCCGAAGCGCACTCGGCAGCTGCCCGTCT
TCGACAGCGGACGCTCCCTCACGCCCGACGAGATGGACGACTCGGTGTACGAGCACATCAAAGAGCGGAACGCACGCCGT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References