81028

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 656271..657070 Replicon chromosome
Accession NZ_CP023377
Organism Escherichia coli strain 127

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag CNQ50_RS03470 Protein ID WP_000347273.1
Coordinates 656271..656735 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag CNQ50_RS03475 Protein ID WP_001307405.1
Coordinates 656735..657070 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ50_RS03435 651272..651706 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
CNQ50_RS03440 651724..652602 - 879 WP_001300474.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
CNQ50_RS03445 652592..653371 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
CNQ50_RS03450 653382..653855 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
CNQ50_RS03455 653878..655158 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
CNQ50_RS03465 655407..656216 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
CNQ50_RS03470 656271..656735 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
CNQ50_RS03475 656735..657070 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
CNQ50_RS03480 657219..658790 - 1572 WP_001723936.1 galactarate dehydratase -
CNQ50_RS03485 659165..660499 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
CNQ50_RS03490 660515..661285 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 656271..667943 11672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T81028 WP_000347273.1 NZ_CP023377:c656735-656271 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T81028 NZ_CP023377:c656735-656271 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT81028 WP_001307405.1 NZ_CP023377:c657070-656735 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT81028 NZ_CP023377:c657070-656735 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References