171852

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 616952..617751 Replicon chromosome
Accession NZ_CP060979
Organism Escherichia coli strain EC3

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H9191_RS03015 Protein ID WP_000347273.1
Coordinates 616952..617416 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9191_RS03020 Protein ID WP_001307405.1
Coordinates 617416..617751 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9191_RS02985 (611953) 611953..612387 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H9191_RS02990 (612405) 612405..613283 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
H9191_RS02995 (613273) 613273..614052 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9191_RS03000 (614063) 614063..614536 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9191_RS03005 (614559) 614559..615839 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9191_RS03010 (616088) 616088..616897 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9191_RS03015 (616952) 616952..617416 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9191_RS03020 (617416) 617416..617751 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9191_RS03025 (617900) 617900..619471 - 1572 WP_001723936.1 galactarate dehydratase -
H9191_RS03030 (619846) 619846..621180 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
H9191_RS03035 (621196) 621196..621966 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 616952..628624 11672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T171852 WP_000347273.1 NZ_CP060979:c617416-616952 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T171852 NZ_CP078139:c5892882-5892592 [Pseudomonas savastanoi pv. savastanoi]
ATGACCTATAACCTCGAATTCGATGCGCGGGCTCTAAAGGAATGGCACAAATTAGGAGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCCAATCGTCTGCATGCATTGCCTGACTGCTACAAAATCA
AACTGCGCAGTAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCGGTAGACAAG
CGTGAACGGGAAGAGGTTTATCGCAAGGCGACGGACCGGCTGGGTAGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171852 WP_001307405.1 NZ_CP060979:c617751-617416 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171852 NZ_CP078139:c5893111-5892872 [Pseudomonas savastanoi pv. savastanoi]
ATGGCGTCTATCAACGTTCGTATCGACGACGATCTCAAAGCGCGTGCTTACCTCGAGCTGGAAAAGCTTGGCGTCACCCC
CTCGGAGTTGCTGCGCCAAACCCTGCAGTACGTAGCTGAGCGTGGTCAGCTACCCTTCAAGACGGTTCTGATGACAGAGG
AAGACGAAGCGCTGTTGGCAACTGTGCGCGAGCGCCTTGCAGCTCCGCAGCGGGTCAAGGTTTCACTGGATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References