269428

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 590442..591241 Replicon chromosome
Accession NZ_CP116923
Organism Escherichia coli strain MLI104K1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag NL419_RS03350 Protein ID WP_000347273.1
Coordinates 590442..590906 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NL419_RS03355 Protein ID WP_001307405.1
Coordinates 590906..591241 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL419_RS03320 (585443) 585443..585877 - 435 WP_072667546.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NL419_RS03325 (585895) 585895..586773 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NL419_RS03330 (586763) 586763..587542 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
NL419_RS03335 (587553) 587553..588026 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
NL419_RS03340 (588049) 588049..589329 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
NL419_RS03345 (589578) 589578..590387 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NL419_RS03350 (590442) 590442..590906 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NL419_RS03355 (590906) 590906..591241 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NL419_RS03360 (591390) 591390..592961 - 1572 WP_001273753.1 galactarate dehydratase -
NL419_RS03365 (593336) 593336..594670 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NL419_RS03370 (594686) 594686..595456 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 590442..601211 10769


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T269428 WP_000347273.1 NZ_CP116923:c590906-590442 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT269428 WP_001307405.1 NZ_CP116923:c591241-590906 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References