Detailed information of TA system TA035065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 605461..606260
• Replicon: chromosome
• Accession: NZ_AP022245
• Organism: Escherichia coli strain WP8-S18-CRE-02

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: H7R30_RS02885
• Protein ID: WP_000347273.1
• Coordinates: 605461..605925 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: H7R30_RS02890
• Protein ID: WP_021547164.1
• Coordinates: 605925..606260 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R30_RS02855	600462..600896	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
H7R30_RS02860	600914..601792	-	879	WP_001300474.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
H7R30_RS02865	601782..602561	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
H7R30_RS02870	602572..603045	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
H7R30_RS02875	603068..604348	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
H7R30_RS02880	604597..605406	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
H7R30_RS02885	605461..605925	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
H7R30_RS02890	605925..606260	-	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
H7R30_RS02895	606409..607980	-	1572	WP_001753084.1	galactarate dehydratase	-
H7R30_RS02900	608355..609689	+	1335	WP_152071808.1	galactarate/glucarate/glycerate transporter GarP	-
H7R30_RS02905	609705..610475	+	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020080 3504819..3516493 0.007 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021455 3265982..3277656 0.007 View NC_009800 Escherichia coli HS Genomic island TA022436 3314965..3326421 0.009 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068442 3158126..3169800 0.007 View NZ_CP020543 Escherichia coli C Genomic island TA075057 640607..652281 0.007 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285025 3504819..3516493 0.007	inside	Genomic island	-	-	605461..617135	11674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T35065 WP_000347273.1 NZ_AP022245:c605925-605461 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T35065 NZ_AP022245:c605925-605461 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGGCATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT35065 WP_021547164.1 NZ_AP022245:c606260-605925 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT35065 NZ_AP022245:c606260-605925 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39