252163

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 624321..625120 Replicon chromosome
Accession NZ_CP101513
Organism Escherichia coli strain HH194M

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag MOO21_RS03030 Protein ID WP_000347273.1
Coordinates 624321..624785 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MOO21_RS03035 Protein ID WP_001307405.1
Coordinates 624785..625120 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO21_RS03000 (619322) 619322..619756 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MOO21_RS03005 (619774) 619774..620652 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
MOO21_RS03010 (620642) 620642..621421 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MOO21_RS03015 (621432) 621432..621905 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MOO21_RS03020 (621928) 621928..623208 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
MOO21_RS03025 (623457) 623457..624266 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MOO21_RS03030 (624321) 624321..624785 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MOO21_RS03035 (624785) 624785..625120 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MOO21_RS03040 (625269) 625269..626840 - 1572 WP_001723936.1 galactarate dehydratase -
MOO21_RS03045 (627215) 627215..628549 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
MOO21_RS03050 (628565) 628565..629335 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 624321..635993 11672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T252163 WP_000347273.1 NZ_CP101513:c624785-624321 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT252163 WP_001307405.1 NZ_CP101513:c625120-624785 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References