Detailed information of TA system TA052035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3589823..3590622
• Replicon: chromosome
• Accession: NZ_CP010315
• Organism: Escherichia coli strain 789

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: AC789_RS17685
• Protein ID: WP_000347251.1
• Coordinates: 3590158..3590622 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: AC789_RS17680
• Protein ID: WP_001307405.1
• Coordinates: 3589823..3590158 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AC789_RS17665	3585608..3586378	-	771	WP_001058214.1	2-dehydro-3-deoxyglucarate aldolase	-
AC789_RS17670	3586394..3587728	-	1335	WP_000599653.1	galactarate/glucarate/glycerate transporter GarP	-
AC789_RS17675	3588103..3589674	+	1572	WP_001273761.1	galactarate dehydratase	-
AC789_RS17680	3589823..3590158	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AC789_RS17685	3590158..3590622	+	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AC789_RS17690	3590677..3591486	-	810	WP_000072192.1	aga operon transcriptional regulator AgaR	-
AC789_RS17695	3591735..3593015	+	1281	WP_044502339.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AC789_RS17700	3593038..3593511	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AC789_RS17705	3593522..3594301	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
AC789_RS17710	3594291..3595169	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
AC789_RS17715	3595187..3595621	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020080 3504819..3516493 0.009 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021455 3265982..3277656 0.009 View NC_009800 Escherichia coli HS Genomic island TA022436 3314965..3326421 0.006 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068442 3158126..3169800 0.009 View NZ_CP020543 Escherichia coli C Genomic island TA075057 640607..652281 0.009 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285025 3504819..3516493 0.009	inside	Genomic island	-	-	3579173..3590622	11449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T52035 WP_000347251.1 NZ_CP010315:3590158-3590622 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T52035 NZ_CP010315:3590158-3590622 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT52035 WP_001307405.1 NZ_CP010315:3589823-3590158 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT52035 NZ_CP010315:3589823-3590158 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: A0A0E0XVC7