Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4306507..4307306 Replicon chromosome
Accession NZ_CP044312
Organism Escherichia coli strain RM13745

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag F7D05_RS21195 Protein ID WP_000347251.1
Coordinates 4306842..4307306 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag F7D05_RS21190 Protein ID WP_001307405.1
Coordinates 4306507..4306842 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7D05_RS21175 4302292..4303062 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
F7D05_RS21180 4303078..4304412 - 1335 WP_000599653.1 galactarate/glucarate/glycerate transporter GarP -
F7D05_RS21185 4304787..4306358 + 1572 WP_001273761.1 galactarate dehydratase -
F7D05_RS21190 4306507..4306842 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
F7D05_RS21195 4306842..4307306 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
F7D05_RS21200 4307361..4308170 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
F7D05_RS21205 4308419..4309699 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
F7D05_RS21210 4309722..4310195 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
F7D05_RS21215 4310206..4310985 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
F7D05_RS21220 4310975..4311853 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
F7D05_RS21225 4311871..4312305 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4295737..4307306 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T140602 WP_000347251.1 NZ_CP044312:4306842-4307306 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T140602 NZ_CP059319:c2611949-2611521 [Rhizorhabdus wittichii]
ATGATCGTCCTCGACACCAATGTCGTCTCCGAGGCGATGAAGCCTGAACCGAACCCGGACGTGTGCGCCTGGCTCGATGA
GCAGGCGGCGGAGACGCTCTATCTTTCGAGCGTGACGATCGCGGAACTGCTGTTCGGTATCGGCGCGCTTCCCGACGGCC
GGCGCAAGGACAGGCTGGCCGTGGCCTTGGACGGGCTGCTCGAGCTGTTCGACGGGCGGATCCTGCCCTTCGACATGGCG
GCCGCCCGTCATTATGCCGATCGCGCGGTCAGGGCGCGCAGTGCCGGTAAGGGATTTCCAACGCCGGACGGATATATCGC
CGCCATCGCGGTCTCCCGCAGCTTCGTCGTGGCGACCCGGGATGCCAGCGCATTCGACGCCGCCGGAATCGCCGTCATCG
ATCCCTGGAAGGCCGATCGGCGGCAATAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT140602 WP_001307405.1 NZ_CP044312:4306507-4306842 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT140602 NZ_CP059319:c2612200-2611946 [Rhizorhabdus wittichii]
ATGCCTGCCGTCACCATCCGCAATCTCTCCGAAGAGGCGCATCGCGCGCTCAAGATTCGGGCCGCCCACCATGGCCGCAG
CACCGAGGCGGAGATGCGCGATATCCTGGAAGGCGCGGTTCGCCCGGCGAATCGCGTTCGTATCGGCAGCGCGCTGTCCC
GGTTGAGCCGCGATGCCGGGCTGACCAATGCCGATTTCGAGGCTCTCGATCAGGCTCGCGACCGCAAGCCCGCCGAACCG
CTGGGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References