191734

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2373271..2374070 Replicon chromosome
Accession NZ_CP069710
Organism Escherichia coli strain EA2

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag JSQ74_RS11395 Protein ID WP_000347251.1
Coordinates 2373606..2374070 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JSQ74_RS11390 Protein ID WP_001307405.1
Coordinates 2373271..2373606 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ74_RS11375 2369056..2369826 - 771 WP_021564856.1 2-dehydro-3-deoxyglucarate aldolase -
JSQ74_RS11380 2369842..2371176 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JSQ74_RS11385 2371551..2373122 + 1572 WP_096261350.1 galactarate dehydratase -
JSQ74_RS11390 2373271..2373606 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JSQ74_RS11395 2373606..2374070 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JSQ74_RS11400 2374125..2374934 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JSQ74_RS11405 2375183..2376463 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
JSQ74_RS11410 2376486..2376959 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JSQ74_RS11415 2376970..2377749 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JSQ74_RS11420 2377739..2378617 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JSQ74_RS11425 2378635..2379069 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2362501..2374070 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T191734 WP_000347251.1 NZ_CP069710:2373606-2374070 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T191734 NZ_CP091892:c2832654-2832313 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACGCGATATACGTTGCCCGATGACATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT191734 WP_001307405.1 NZ_CP069710:2373271-2373606 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT191734 NZ_CP091892:c2832884-2832654 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References