Detailed information of TA system TA072207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3411059..3411858
• Replicon: chromosome
• Accession: NZ_CP019280
• Organism: Escherichia coli strain 13P484A

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: BWI90_RS17965
• Protein ID: WP_148119790.1
• Coordinates: 3411394..3411858 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: BWI90_RS17960
• Protein ID: WP_001307405.1
• Coordinates: 3411059..3411394 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI90_RS17945	3406844..3407614	-	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-
BWI90_RS17950	3407630..3408964	-	1335	WP_001723935.1	galactarate/glucarate/glycerate transporter GarP	-
BWI90_RS17955	3409339..3410910	+	1572	WP_001723936.1	galactarate dehydratase	-
BWI90_RS17960	3411059..3411394	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
BWI90_RS17965	3411394..3411858	+	465	WP_148119790.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
BWI90_RS17970	3411913..3412722	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
BWI90_RS17980	3412971..3414251	+	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
BWI90_RS17985	3414274..3414747	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
BWI90_RS17990	3414758..3415537	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
BWI90_RS17995	3415527..3416405	+	879	WP_001300474.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
BWI90_RS18000	3416423..3416857	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_000913 Escherichia coli str. K-12 substr. MG1655 Genomic island TA001047 3267854..3277801 0.01 View NC_010473 Escherichia coli str. K-12 substr. DH10B Genomic island TA022703 3363621..3373568 0.01 View NC_012759 Escherichia coli BW2952 Genomic island TA023871 3153024..3162971 0.01 View NC_020518 Escherichia coli str. K-12 substr. MDS42 Genomic island TA029016 2741187..2751134 0.01 View NZ_CP009273 Escherichia coli BW25113 Genomic island TA048700 3261213..3271160 0.01	inside	Genomic island	-	-	3402025..3411858	9833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17848.31 Da
• Isoelectric Point: 9.6924

>T72207 WP_148119790.1 NZ_CP019280:3411394-3411858 [Escherichia coli]
MDFPQRVNGWALYAHPCFQEIYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T72207 NZ_CP019280:3411394-3411858 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAATCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT72207 WP_001307405.1 NZ_CP019280:3411059-3411394 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT72207 NZ_CP019280:3411059-3411394 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0XVC7