Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4560918..4561693 Replicon chromosome
Accession NC_016845
Organism Klebsiella pneumoniae subsp. pneumoniae HS11286

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A0H3GXP2
Locus tag KPHS_45340 Protein ID YP_005228834.1
Coordinates 4560918..4561334 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0H3GVL4
Locus tag KPHS_45350 Protein ID YP_005228835.1
Coordinates 4561400..4561693 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPHS_45300 4556087..4557454 - 1368 YP_005228830.1 replicative DNA helicase -
KPHS_45310 4557830..4558702 - 873 YP_005228831.1 chromosome partitioning ATPase -
KPHS_45320 4559372..4559524 - 153 YP_005228832.1 transposase -
KPHS_45330 4559621..4560214 - 594 YP_005228833.1 hypothetical protein -
KPHS_45340 4560918..4561334 - 417 YP_005228834.1 GNAT family N-acetyltransferase Toxin
KPHS_45350 4561400..4561693 - 294 YP_005228835.1 putative CopG family helix-turn-helix protein Antitoxin
KPHS_45360 4562338..4563714 + 1377 YP_005228836.1 cystathionine-beta-synthase -
KPHS_45370 4563725..4564873 + 1149 YP_005228837.1 cystathionine-gamma-lyase -
KPHS_45380 4564874..4565785 - 912 YP_005228838.1 LysR family transcriptional regulator -
KPHS_45390 4565883..4566485 + 603 YP_005228839.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4502831..4561944 59113
flank IS/Tn - - 4559372..4559524 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(18-120)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15159.79 Da        Isoelectric Point: 10.2776

>T26030 YP_005228834.1 NC_016845:c4561334-4560918 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPIPVVVLGRLAVDKSLHGKGVGRAL
VRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVNA

Download         Length: 417 bp

>T26030 NC_016845:c4561334-4560918 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGGATCACTGGCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGC
GAAGGTGATGGCTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGC
CTGCCCCGATCCCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTG
GTACGTGATGCCGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGA
TGAAGCACGGGATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGG
ATTTGGTTAATGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT26030 YP_005228835.1 NC_016845:c4561693-4561400 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT26030 NC_016845:c4561693-4561400 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GXP2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References