Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 789712..790487 Replicon chromosome
Accession NZ_CP051160
Organism Klebsiella pneumoniae strain KP20191015

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HF573_RS04000 Protein ID WP_004150910.1
Coordinates 790002..790487 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HF573_RS03995 Protein ID WP_004150912.1
Coordinates 789712..790005 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF573_RS03975 784920..785522 - 603 WP_002916735.1 short chain dehydrogenase -
HF573_RS03980 785620..786531 + 912 WP_004188550.1 LysR family transcriptional regulator -
HF573_RS03985 786532..787680 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HF573_RS03990 787691..789067 - 1377 WP_004217775.1 cystathionine beta-synthase -
HF573_RS03995 789712..790005 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HF573_RS04000 790002..790487 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HF573_RS04005 791191..791783 + 593 Protein_788 hypothetical protein -
HF573_RS04010 791880..792096 + 217 Protein_789 transposase -
HF573_RS04015 792896..793756 + 861 WP_000158645.1 hypothetical protein -
HF573_RS04020 793972..794220 + 249 WP_000616796.1 AlpA family transcriptional regulator -
HF573_RS04025 794217..794528 + 312 WP_000837631.1 hypothetical protein -
HF573_RS04030 794543..794929 + 387 WP_001545719.1 hypothetical protein -
HF573_RS04035 794946..795233 + 288 WP_001291073.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 791880..792032 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T153636 WP_004150910.1 NZ_CP051160:790002-790487 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T153636 NZ_CP066997:4969134-4969289 [Xanthomonas campestris pv. campestris]
GTGACCCTGCTCGATACCAATGTCATCTTCGGGCCCAATCTGCATGTCGTCGCCTGGATCGATGTGCAGGCCGTTGAAAC
ACTGTTCCTGTCGGTCGTGACGGTGGCCGAATTGCGATCTGGAGACATTCAGGATTTCAGACCCCTGATGAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT153636 WP_004150912.1 NZ_CP051160:789712-790005 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT153636 NZ_CP066997:4968883-4969137 [Xanthomonas campestris pv. campestris]
ATGCCTTCTTTCACCGTCCGCAACATCCCGGAGGATGTGCATCGCGCCATCTGGGCACGTGCTGCCCAACACGGGCGCAG
CACCGAGGCGGAGATCCGCGCGATCCTTGAATCAGCAGCCAAGCCAGCCGACCAGGTCAAACTTGGCGCGTTGCTGGTGG
ACATCGGCCGCGACGCTGGCCTGACAGCAAAAGAGGCCGACGCCTTCGCCAAGTTACGCGACAAGGCCGCGATCAAGCCG
ATCGGTTTGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References