Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 746510..747285 Replicon chromosome
Accession NZ_CP082840
Organism Klebsiella pneumoniae strain W09308

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag K6041_RS03730 Protein ID WP_004150910.1
Coordinates 746800..747285 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K6041_RS03725 Protein ID WP_004150912.1
Coordinates 746510..746803 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6041_RS03705 (K6041_03700) 741718..742320 - 603 WP_002916735.1 short chain dehydrogenase -
K6041_RS03710 (K6041_03705) 742418..743329 + 912 WP_004188550.1 LysR family transcriptional regulator -
K6041_RS03715 (K6041_03710) 743330..744478 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
K6041_RS03720 (K6041_03715) 744489..745865 - 1377 WP_004217775.1 cystathionine beta-synthase -
K6041_RS03725 (K6041_03720) 746510..746803 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K6041_RS03730 (K6041_03725) 746800..747285 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
K6041_RS03735 (K6041_03730) 747989..748582 + 594 WP_004188553.1 hypothetical protein -
K6041_RS03740 (K6041_03735) 748679..748895 + 217 Protein_735 transposase -
K6041_RS03745 (K6041_03740) 749501..750373 + 873 WP_004188557.1 ParA family protein -
K6041_RS03750 (K6041_03745) 750373..750756 + 384 WP_004150906.1 hypothetical protein -
K6041_RS03755 (K6041_03750) 750749..752116 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 748679..748831 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T215405 WP_004150910.1 NZ_CP082840:746800-747285 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T215405 NZ_CP110674:1742707-1742955 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT215405 WP_004150912.1 NZ_CP082840:746510-746803 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT215405 NZ_CP110674:1742495-1742707 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References