238668

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4418360..4419135 Replicon chromosome
Accession NZ_CP093465
Organism Klebsiella pneumoniae strain VA681

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MO412_RS21655 Protein ID WP_004150910.1
Coordinates 4418360..4418845 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MO412_RS21660 Protein ID WP_004150912.1
Coordinates 4418842..4419135 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO412_RS21630 (MO412_21610) 4413529..4414896 - 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -
MO412_RS21635 (MO412_21615) 4414889..4415272 - 384 WP_004150906.1 hypothetical protein -
MO412_RS21640 (MO412_21620) 4415272..4416144 - 873 WP_004188557.1 ParA family protein -
MO412_RS21645 (MO412_21625) 4416750..4416966 - 217 Protein_4227 transposase -
MO412_RS21650 (MO412_21630) 4417063..4417656 - 594 WP_004188553.1 hypothetical protein -
MO412_RS21655 (MO412_21635) 4418360..4418845 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MO412_RS21660 (MO412_21640) 4418842..4419135 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MO412_RS21665 (MO412_21645) 4419780..4421156 + 1377 WP_004217775.1 cystathionine beta-synthase -
MO412_RS21670 (MO412_21650) 4421167..4422315 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MO412_RS21675 (MO412_21655) 4422316..4423227 - 912 WP_004188550.1 LysR family transcriptional regulator -
MO412_RS21680 (MO412_21660) 4423325..4423927 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 4358832..4419386 60554
flank IS/Tn - - 4416814..4416966 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T238668 WP_004150910.1 NZ_CP093465:c4418845-4418360 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T238668 NZ_LR890430:69663-69812 [Klebsiella pneumoniae]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT238668 WP_004150912.1 NZ_CP093465:c4419135-4418842 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT238668 NZ_LR890430:c69618-69559 [Klebsiella pneumoniae]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References