Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 742092..742867 Replicon chromosome
Accession NZ_CP047649
Organism Klebsiella pneumoniae strain 158590

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag GTK59_RS03680 Protein ID WP_004150910.1
Coordinates 742382..742867 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag GTK59_RS03675 Protein ID WP_004150912.1
Coordinates 742092..742385 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK59_RS03655 737300..737902 - 603 WP_062954968.1 short chain dehydrogenase -
GTK59_RS03660 738000..738911 + 912 WP_004188550.1 LysR family transcriptional regulator -
GTK59_RS03665 738912..740060 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
GTK59_RS03670 740071..741447 - 1377 WP_004217775.1 cystathionine beta-synthase -
GTK59_RS03675 742092..742385 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
GTK59_RS03680 742382..742867 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
GTK59_RS03685 743571..744164 + 594 WP_004188553.1 hypothetical protein -
GTK59_RS03690 744261..744477 + 217 Protein_732 transposase -
GTK59_RS03705 745083..745955 + 873 WP_004188557.1 ParA family protein -
GTK59_RS03710 745955..746338 + 384 WP_004150906.1 hypothetical protein -
GTK59_RS03715 746331..747698 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 744261..744413 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T147047 WP_004150910.1 NZ_CP047649:742382-742867 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T147047 NZ_CP062853:c4163853-4163680 [Escherichia coli O9:H10]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT147047 WP_004150912.1 NZ_CP047649:742092-742385 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT147047 NZ_CP062853:c4164177-4163881 [Escherichia coli O9:H10]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References