Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4586880..4587655 Replicon chromosome
Accession NZ_CP095428
Organism Klebsiella pneumoniae strain IM007

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MWR71_RS22625 Protein ID WP_004150910.1
Coordinates 4587170..4587655 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MWR71_RS22620 Protein ID WP_004150912.1
Coordinates 4586880..4587173 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR71_RS22600 (MWR71_22570) 4582088..4582690 - 603 WP_062954968.1 short chain dehydrogenase -
MWR71_RS22605 (MWR71_22575) 4582788..4583699 + 912 WP_004188550.1 LysR family transcriptional regulator -
MWR71_RS22610 (MWR71_22580) 4583700..4584848 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MWR71_RS22615 (MWR71_22585) 4584859..4586235 - 1377 WP_004217775.1 cystathionine beta-synthase -
MWR71_RS22620 (MWR71_22590) 4586880..4587173 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MWR71_RS22625 (MWR71_22595) 4587170..4587655 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MWR71_RS22630 (MWR71_22600) 4588359..4588952 + 594 WP_004188553.1 hypothetical protein -
MWR71_RS22635 (MWR71_22605) 4589049..4589265 + 217 Protein_4427 transposase -
MWR71_RS22640 (MWR71_22610) 4589871..4590743 + 873 WP_004188557.1 ParA family protein -
MWR71_RS22645 (MWR71_22615) 4590743..4591126 + 384 WP_004150906.1 hypothetical protein -
MWR71_RS22650 (MWR71_22620) 4591119..4592486 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4589049..4589201 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T241993 WP_004150910.1 NZ_CP095428:4587170-4587655 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T241993 NZ_OU943336:805351-805752 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCCTTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGACAG
GACTGGACGCTCTTGCGTCCTCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
AG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT241993 WP_004150912.1 NZ_CP095428:4586880-4587173 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT241993 NZ_OU943336:805104-805370 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References