Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 740540..741315 Replicon chromosome
Accession NZ_CP082010
Organism Klebsiella pneumoniae strain KP18-41

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag K6147_RS03745 Protein ID WP_004150910.1
Coordinates 740830..741315 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K6147_RS03740 Protein ID WP_004150912.1
Coordinates 740540..740833 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6147_RS03720 735748..736350 - 603 WP_062954968.1 short chain dehydrogenase -
K6147_RS03725 736448..737359 + 912 WP_004188550.1 LysR family transcriptional regulator -
K6147_RS03730 737360..738508 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
K6147_RS03735 738519..739895 - 1377 WP_004217775.1 cystathionine beta-synthase -
K6147_RS03740 740540..740833 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K6147_RS03745 740830..741315 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
K6147_RS03750 742019..742612 + 594 WP_004188553.1 hypothetical protein -
K6147_RS03755 742709..742925 + 217 Protein_729 transposase -
K6147_RS03760 743531..744403 + 873 WP_004188557.1 ParA family protein -
K6147_RS03765 744403..744786 + 384 WP_004150906.1 hypothetical protein -
K6147_RS03770 744779..746146 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 742709..742861 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T213888 WP_004150910.1 NZ_CP082010:740830-741315 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T213888 NZ_CP109897:c1217345-1216734 [Xylella fastidiosa subsp. fastidiosa]
ATGAAATACGCAGGGGATCGTGGTGATCCTTACCTGGACAGCGAAACAGGTGTTCTTCGTAATCTCCTTGGAATCAGGGA
TCAGGGGGGGCTCGATAAAATAGAGTCCACCCTTTCTTTTTTGCGAACCAGCGAATTGCGTGAGCGACCCGTAAAAGGCA
AATTTGATCTAGCGCATTTACAGGAAATCCATAATCGTCTTTTCCAGGATGTGTACGACTGGGCGGGTCAAATCCGTCAA
GTTGAAATCTCGAAAGGCAACACGATGTTTGCCCAGCAGATTGCGATTCAGAGTGCGGCGCAGCAGATCTTTGGGCAGCT
TGCTAAAGAGCGATTTTTGTGTGGCCTTGATGCTGAAGAATTCAGCAAACGGGCAGGTGATTATCTTGGTGAAATTAATG
TGCTTCACCCATTCCGCGAGGGAAATGGCAGGACGCAACGGGAATTCATCGCGCAATTGGCTCAGCGTGCAGGTTACCGG
ATCGACTGGGGCGTGGTCAGCCAGGCGGATATGATTAAAGCGTCAATCGACGCTTACAACGGGGATTCAAGTGGATTGGC
TAGTATCATCCGCGAAGGAATATCTGATCAGCTTTTTAAAGATAATGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT213888 WP_004150912.1 NZ_CP082010:740540-740833 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT213888 NZ_CP109897:c1217530-1217342 [Xylella fastidiosa subsp. fastidiosa]
ATGCTGACAGATCATGAGAAAGCTGAACGCCGTGCTGTGGTGCAAAGCGCACTCGCAAGCCAACGCATTGAGGGGTTAGA
GCCGGATGCGCAGGCCGTGGTTGATGCTGAATGCTGGGCGCGTGGTGAAATGACTATCGCTGCCGCCGTGGACCAGTACA
AAGCGCGTGTTCGGCTCCAGATTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References