Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 738917..739692 Replicon chromosome
Accession NZ_CP063008
Organism Klebsiella pneumoniae strain CriePir120

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag GJJ08_RS03685 Protein ID WP_004150910.1
Coordinates 739207..739692 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag GJJ08_RS03680 Protein ID WP_004150912.1
Coordinates 738917..739210 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJJ08_RS03660 734125..734727 - 603 WP_002916735.1 short chain dehydrogenase -
GJJ08_RS03665 734825..735736 + 912 WP_004188550.1 LysR family transcriptional regulator -
GJJ08_RS03670 735737..736885 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
GJJ08_RS03675 736896..738272 - 1377 WP_004217775.1 cystathionine beta-synthase -
GJJ08_RS03680 738917..739210 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
GJJ08_RS03685 739207..739692 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
GJJ08_RS03690 740396..740989 + 594 WP_004188553.1 hypothetical protein -
GJJ08_RS03695 741086..741302 + 217 Protein_726 transposase -
GJJ08_RS03700 741908..742780 + 873 WP_004188557.1 ParA family protein -
GJJ08_RS03705 742780..743163 + 384 WP_004150906.1 hypothetical protein -
GJJ08_RS03710 743156..744523 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 741086..741238 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T177465 WP_004150910.1 NZ_CP063008:739207-739692 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T177465 NZ_CP083497:c2375932-2375558 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTTGG
CCGTCTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT177465 WP_004150912.1 NZ_CP063008:738917-739210 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT177465 NZ_CP083497:c2376390-2376022 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACTCATCCCGATGACAACAACAGCCACCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAGCTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTTACCTGCAAAGCCGATACCCTCGGCAGTCG
AGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References