Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3094942..3095717 Replicon chromosome
Accession NZ_CP095234
Organism Klebsiella pneumoniae strain 21072329

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag MU289_RS15095 Protein ID WP_004150910.1
Coordinates 3095232..3095717 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag MU289_RS15090 Protein ID WP_004150912.1
Coordinates 3094942..3095235 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU289_RS15070 (MU289_15070) 3090150..3090752 - 603 WP_062954968.1 short chain dehydrogenase -
MU289_RS15075 (MU289_15075) 3090850..3091761 + 912 WP_004188550.1 LysR family transcriptional regulator -
MU289_RS15080 (MU289_15080) 3091762..3092910 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
MU289_RS15085 (MU289_15085) 3092921..3094297 - 1377 WP_004217775.1 cystathionine beta-synthase -
MU289_RS15090 (MU289_15090) 3094942..3095235 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
MU289_RS15095 (MU289_15095) 3095232..3095717 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
MU289_RS15100 (MU289_15100) 3096421..3097014 + 594 WP_004188553.1 hypothetical protein -
MU289_RS15105 (MU289_15105) 3097111..3097327 + 217 Protein_2942 transposase -
MU289_RS15110 (MU289_15110) 3097933..3098805 + 873 WP_004188557.1 ParA family protein -
MU289_RS15115 (MU289_15115) 3098805..3099188 + 384 WP_004150906.1 hypothetical protein -
MU289_RS15120 (MU289_15120) 3099181..3100548 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3097111..3097263 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T241708 WP_004150910.1 NZ_CP095234:3095232-3095717 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T241708 NZ_OD940437:c1524971-1524108 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTCACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTTATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GATTAGAAACGCCTTCTATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGCTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT241708 WP_004150912.1 NZ_CP095234:3094942-3095235 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT241708 NZ_OD940437:c1525245-1524973 [Enterococcus faecalis]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References